Certificate Course module

1.

Introduction of Plant tissue culture and basic knowledge about setting up Plant tissue culture laboratory

1st

1.1

Working of  various equipments and their safe handling

2.

Basic theoretical knowledge of  micro and macro nutrients required for plant tissue culture medium.

2nd

2.1

Preparation of  stocks solutions, hormones required for nutrient media 

3.

Theory about different plant tissue culture nutrient medium and its components.

3rd

3.1

Preparation of   basal/specific nutrient MS medium for commercially important plant species with details of procedure and its sterilization by autoclaving

4.

Theory about methodologies  and optimized protocols in JUIT lab for tissue culture

4th

4.1

Surface sterilization and  establishment of tissue cultures by using shoot apex

5.

Theory about maintenance and analysis of growth parameters of shoot culture

5th

5.1

Optimization of incubation conditions and observation of growth of plantlets

Mid Term Evaluation  (20 Maximum Marks)

6th

6.

Theory about callus cultures with significance

7th

6.1

Media preparation for callus induction  and regeneration

7.

Micro propagation of different plant species by using different explants for large scale shoot proliferation

8th

7.1

Incubation of cultures under optimized culture room conditions and observation of growth parameters

8.

Theory about in vitro and ex vitro root induction

9th

8.1

Media preparation for in vitro root induction

9.

Theory about different articles and setups for hardening of tissue cultured plants in green house

10th

9.1

Transfer of plants  for hardening and ex vitro rooting under green house conditions

10.

Theory about hydroponic systems

11th

10.2

Media preparation  and establishment of plants under the hydroponic system

11.

Theory about  production of phyto chemicals

12th

11.1

Establishment of  cell/tissue culture systems for production of phytochemicals

Final Evaluation

13th